CleaScrip T7 RNA Polymerase (Low dsRNA, 250 U/μL) is a high-efficiency enzyme specifically optimized for in vitro transcription of high-purity RNA with minimal double-stranded RNA (dsRNA) byproducts. Its low-dsRNA formulation reduces unwanted immune activation, making it ideal for sensitive applications such as mRNA vaccine development, CRISPR guide RNA synthesis, in vitro translation, and RNA probe generation. With a powerful concentration of 250 U/μL, CleaScrip™ ensures exceptional yields, high transcription fidelity, and reproducible performance across research and bioproduction workflows. Designed for templates containing a T7 promoter, this enzyme delivers consistent, clean RNA suitable for both laboratory research and therapeutic manufacturing.

This product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyses the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.

The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA. Note: G* is the first base of the RNA transcript. This product is produced in accordance with GMP regulations and provided in liquid form.

Feature Synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template Higher yield and easy to operate Lower endotoxins Tested for the absence of endonucleases, exonucleases, RNases Application Radiolabelled RNA probe preparation RNA generation for studies of RNA structure, processing and catalysis Expression control via anti-sense RNA mRNA, sgRNA synthesis

Note: Cleascript is a wrong denomination of Cleascrip also found in NCBI publications.